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2 edition of Studies on the glycosphingolipids of normal and transformed cultured mammalian cells found in the catalog.

Studies on the glycosphingolipids of normal and transformed cultured mammalian cells

Ganesa Yogeeswaran

Studies on the glycosphingolipids of normal and transformed cultured mammalian cells

  • 165 Want to read
  • 21 Currently reading

Published in [Toronto] .
Written in English

    Subjects:
  • Glycosphingolipids,
  • Sphingolipids

  • Edition Notes

    ContributionsToronto, University.
    The Physical Object
    Paginationxi, 173 leaves.
    Number of Pages173
    ID Numbers
    Open LibraryOL18907206M

    Differentiated cells can be reprogrammed to an embryonic-like state by transfer of nuclear contents into oocytes or by fusion with embryonic stem (ES) cells. Little is known about factors that induce this reprogramming. Here, we demonstrate induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell Cited by: A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Miami Winter Symposia, Volume From Gene to Protein: Information Transfer in Normal and Abnormal Cells presents the expression and processing of genetic information at the levels of both proteins and nucleic acids. This book deals with the reassembly and mobilization of genetic information. Cell culture is one of the major tools used in cellular and molecular biology, providing excellent model systems for studying the normal physiology and biochemistry of cells (e.g., metabolic studies, aging), the effects of drugs and toxic compounds on the cells, and mutagenesis and carcinogenesis.

    Get this from a library! Normal, Transformed and Leukemic Leukocytes: a Scanning Electron Microscopy Atlas. [Aaron Polliack] -- The scanning electron microscope (SEM) has been used with increasing frequency in recent years to study the surface mor phology of normal, transformed and malignant leukocytes. Since the original.


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Studies on the glycosphingolipids of normal and transformed cultured mammalian cells by Ganesa Yogeeswaran Download PDF EPUB FB2

Studies on the Glycosphingolipids of Normal and Virally Transformed 3T3 Mouse Fibroblasts* (Received for publication, Ma ) G. YOGEESTVARAN, ROSE SHEININ, JOHN R. WHERRETT, AND ROBERT IMURRAY$ From the Departments of Biochemistry, Medical Biophjysics, Medicine, and Pathology, University of Torotzto.

Publisher Summary Mammalian cells show a marked simplification of glycolipids when transformed spontaneously or by oncogenic DNA or RNA viruses. This chapter discusses a study of glycosphingolipids in cultured cell lines from human colonic tumors and fetal : Bader Siddiqui, Young S. Kim. Glycosphingolipids (GSLs) are a type of glycolipid.

They are found in the cell membranes of organisms from bacteria to man, and are the major glycans of the vertebrate brain, where more than 80% of glycoconjugates are in the form of glycolipids.

The emphasis of this chapter is on vertebrate glycosphingolipids. Studies on the glycosphingolipids of normal and transformed cultured mammalian cells book   Glycosphingolipids of normal and leukemic human leukocytes. Macher BA, Lee WM, Westrick MA. We have reviewed the studies on neutral glycosphingolipids and gangliosides of normal and leukemia human leukocytes.

In this review, we examine (a) the glycosphingolipid composition of various leukocyte populations, (b) the differences in Cited by: THE JOURNAL OF BIOLOGPXL CHEMISTRY Vol.

No. 1, Issue of Janu pp.Printed in U.S.A. SUMMARY Studies have been carried out on the glycosphingolipids of human KB cells grown in monolayer and suspension culture, and by synchronization of the latter with a double thymidine (2 mM) block.

characterized glycosphingolipids, the application of fast-atom-bombardment mass spectrometry to structural studies, and the evolving role of gangliosides on the surface of mammalian cells in the regulation of cell growth. STRUCTURE The hydrophobic lipid moiety of glycosphingolipids that is anchored in the plasma membrane of.

THOMAS W. KEENAN, STUART PATTON, in Handbook of Milk Composition, 3. Glycosphingolipids. Glycosphingolipids, relatively minor constituents of the MLGM (Table I), have been the subject of a number of investigations over the past several attention has been due to the recognition that certain glycosphingolipids and products of glycosphingolipid catabolism have.

In the first study, the structural binding requirements of Helicobacter pylori BabA adhesin revealed a different carbohydrate binding potential previously definedthan. Adhesion of H.

pylori generalist, specialist and BabA deletion mutant strains were examined using mixtures of Size: 1MB. PBMCs were cultured with or without 10 μM NB-DNJ in CM for 24 hours. Studies on the glycosphingolipids of normal and transformed cultured mammalian cells book CD4 + T cells were then negatively isolated and resuspended in CM with autologous B cells negatively isolated from a freshly thawed aliquot.

T and B cells were cocultured for 7 days with or without anti-CD3/CD28 (1 μg/ml each) and with or without NB-DNJ (10 μM).Cited by:   The Radiobiology of Cultured Mammalian CellS. Mortimer M. Elkind and Gordon F. Whitmore. Prepared under the direction of the American Institute of Biological Sciences for the U.S.

Atomic Energy Commission. Gordon and Breach, New York, xvi Author: W. Sinclair. Glycosphingolipids: Synthesis and functions. inhibited by CS vs. MF in this study. Glycosphingolipids manner in the secretory pathway of mammalian cells with the.

vitro mammalian cell culture and to provide an appreciation of the impact that cell culture technology has on the health and well-being of mankind. Being a large topic area, this review focuses on Studies on the glycosphingolipids of normal and transformed cultured mammalian cells book biotechnology of cell culture: use of mammalian cells to produce products or the culture of cells as products in their own right.

Glycosphingolipids are minor components of cell membranes Studies on the glycosphingolipids of normal and transformed cultured mammalian cells book membrane compartments, and are connected to cell growth, differentiation, and viral and oncogenic transformations.

Like GPI anchored proteins, glycosphingolipids aggregate to form membrane rafts on the micromembrane and the outer leaflet of the apical membrane.

Studies on glycosphingolipids in regenerative medicine 10 Every living cell studied so far, including pluripotent stem cells, are covered by a complex network of carbohydrates often referred to as the glycocalyx [8]. This ultrastructure is formed by glycans covalently bound to proteins or.

Fifteen Years since the Development of KRN – Structure-Activity Relationship Studies on Novel Glycosphingolipids Which Stimulate Natural Killer T Cells Article Jan Author: Suvarn S Kulkarni.

Abstract. Plasma membranes of mammalian cells have been characterized by their higher content of glycosphingolipids (Weinstein et al., ; Dod and Gray, ; Klenk and Choppin, ; Renkonen et al., ; Yogeeswaran et al., ; Critchley et al., ) and by the presence of a particular glycoprotein, as indicated by the higher incorporation of radioactive fucose or glucosamine into Cited by: Both cell and organ culture have applications in pathology, e.g., for comparative, developmental, and diagnostic studies of tissues from normal and diseased donors, for investigations on carcinogenesis, somatic cell genetic variation, viral susceptibility, etc.

Cell cultures are widely used in microbiological studies, for investigations of the. The fatty acid composition of mammalian cells with respect to cellular storage lipids and membrane phospholipids reflects that of lipids present in the culture medium. Modification of membrane phospholipid fatty acyl groups leads to differences in cell function (membrane enzyme activity and transport properties).

Mammalian cells show a marked simplification of glycolipids when transformed spontaneously or by oncogenic DNA or RNA viruses. This chapter discusses a study of glycosphingolipids in cultured cell lines from human colonic tumors and fetal intestines.

Glycosphingolipids were also characterized by MALDI-TOF/TOF MS of oligosaccharides released from glycosphingolipids by enzyme treatment in a highly ambitious study of the total cellular glycome (glycosaminoglycans, N- and O-linked saccharides, and glycosphingolipids) of human pluripotent stem cell lines (hESC and hiPSC) [].However, in this study the cells were cultured on mouse feeder by: 4.

Induction of a Deoxycytidineless State in Cultured Mammalian Cells by Bromodeoxyuridine Expression of Thymidine Kinase Variants is a Function of the Replicative State of Cells Book Reviews Among the Protozoologists Cell Culture-The State of the Art Ribonucleic Acids in Review J.

Bishop. Alterations of Galactosaminyl- and Galactosyltransferases in Cultured Mammalian Cells and in and Grimes, W. J.,Cell surface glycolipid and glycoprotein glycosyltransferases of normal and transformed cells, J. Biol Brady R.O., Fishman P.H.

() Alterations of Galactosaminyl- and Galactosyltransferases in Cultured Cited by: 1. In vitro Studies of the Invasiveness of Cultured Malignant, "Spontaneously" Transformed and Normal Human Bladder Cells P.

Don, J. Kieler* and M. Vilien* Ben Gurion Univ., School of Med., Beersheba, *The Fibiger Laboratory, Copenhagen O, Bladder Cells Israel Denmark ABSTRACT The of ability heart T24 and of 7 human cell was cell lines to invade The and and HU HU bladder into 3 cultured Cited by: 4.

In studies focused on the transformed cells, it was also noted that those cells were able to form a multilayer on top of normal cells.

It was also argued that the loss of contact inhibition was correlated with tumorigenicity. The transformed cells were able to anchorage‐independent growth and proliferate in the absence of serum in medium Cited by: 6.

In general, the hexosamine-containing macromolecules of eukaryotic cells may be classified as glycosphingolipids, glycoproteins, and glycosaminoglycans. Since there is no available information concerning the synthesis of glycoproteins by clonal lines derived from the nervous system in tissue culture, the metabolism of this class of substances Cited by: Start studying cell culture quiz 1.

Learn vocabulary, terms, and more with flashcards, games, and other study tools. name four characteristics of mammalian cell culture. 1) eukaryotic cells are more difficult than prokaryotic cells these changed from normal cells to cells with many of the properties of cancer cells.

some have actually. Given the findings of this study and published work on renal glycosphingolipids, the results suggest the involvement of glycosphingolipids in the deterioration of renal function during aging. Read this article to learn about cell transformation and general characteristics of transformed cells.

The four aspects of Cell Transformation are: (1) Genetic Instability (2) Immortalization (3) Aberrant Growth Control and (4) Tumorigenicity.

Cell transformation due to changes in the genetic material, and cell cloning involving the production of a population single cell are described here. Basics. Glycosphingolipids (GSLs), amphipathic compounds consisting of sugar and Ceramide(Cer) moieties, are ubiquitous components of the plasma membrane of all vertebrate cells.

GSLs are considered to be receptors for bacteria, viruses and their toxins (e.g. Vibrio cholera, HIV), modulators of cell growth and differentiation, organizers of cellular attachment to matrices, play roles in cell. Cell density-dependent changes of glycosphingolipid biosynthesis in cultured human skin fibroblasts.

Glycolipid synthesis in normal and virus-transformed hamster cells, Proc Natl Acad – (). Glycosphingolipids in cultured human skin fibroblasts, J Biol Chem– ().Cited by: Cell Culture Cell culture is one of the major tools used in cellular and molecular biology, providing excellent model systems for studying the normal physiology and biochemistry of cells (e.g., metabolic studies, aging), the effects of drugs and toxic compounds on the cells.

Cell culture is the process by which cells are grown under controlled conditions, generally outside their natural environment. After the cells of interest have been isolated from living tissue, they can subsequently be maintained under carefully controlled conditions vary for each cell type, but generally consist of a suitable vessel with a substrate or medium that supplies.

Vitamin E caused a generalized reduction in the metabolism and cell surface expression of glycosphingolipids (GSL) in cultured Kirsten murine sarcoma virus-transformed nonproducer (K3T3) cells. Metabolism of gangliosides was decreased two- to fourfold in cells treated for 72 hr with 1 and 2 micrograms/ml but not with 12 micrograms/ml vitamin E Cited by: 8.

cells is highly dynamic and is strictly linked to the cellular developmental and environmental state. In the present review, we discuss some of the available knowledge, open questions and future perspectives relating to the study of GSL biology. Introduction Inthe British archaeologist Alexander Cunning.

C6-NBD-ceramide was incorporated into small unilamellar dioleoyl phosphatidylcholine vesicles and incubated with cells in monolayer culture at 2 degrees C, resulting in rapid and preferential transfer of the labeled ceramide from vesicles to cells.

The cells were then washed and subsequently incubated at 37 degrees C for various by: The organization of the plasma membrane of mammalian cells: structure in relation to function. , DOI: /B W. Grimes, Gary A. Van Nest, Arthur R. Kamm. Chemical and immunological studies of cell surfaces from normal and transformed cells.

∗ Cells are cultured as anchorage dependent or independent ∗ Cell lines derived from normal tissues are considered as anchorage-dependent grows only on a suitable substrate e.g. tissue cells ∗ Suspension cells are anchorage- independent e.g.

blood cells ∗ Transformed cell lines either grows as monolayer or as suspension Culturing of cells. Glycosphingolipids are a subtype of glycolipids containing the amino alcohol may be considered as sphingolipids with an attached phingolipids are a group of lipids (more specifically, sphingolipids) and are a part of the cell consist of a hydrophobic ceramide part and a glycosidically bound carbohydrate part.

Invitro Transformation of Cells • Transformed, Infinite or Established Cells • Changed from normal cells to cells with many of the properties of cancer cells • Some of these cell lines have actually been derived from tumors or are transformed spontaneously in culture by mutations • Chemical or gamma ray treated cells can become infinite.

A typical growth curve for cultured cells displays a sigmoid pattern of proliferation. The growth phases associated with normal cells are defined as: Lag Phase – at this stage the cells do not divide. During this period the cells adapt to the culture conditions and the length of this phase will depend upon the growth phase of the cell line at.

Cells need an isotonic environment and pdf plasma osmotic pressure is about mOsm/kg, which is thought to be ideal osmotic pressure to culture human cells. Mouse plasma osmotic pressure is about mOsm/kg. Osmotic pressure of mOsm/kg fits for most mammalian by: Cell culture is one of the major download pdf used in cellular and molecular biology, providing excellent model systems for studying the normal physiology and biochemistry of cells (e.g., metabolic studies, aging), the effects of drugs and toxic compounds on the cells, and mutagenesis and carcinogenesis.

It is also used in drug screening and.COVID Resources. Reliable information about the coronavirus (COVID) is available from ebook World Health Organization (current situation, international travel).Numerous and frequently-updated resource results are available from this ’s WebJunction has pulled together information and resources to assist library staff as they consider how to handle coronavirus.